Temperature-Tolerant COLD-PCR Reduces Temperature Stringency and Enables Robust Mutation Enrichment
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چکیده
منابع مشابه
Temperature-tolerant COLD-PCR reduces temperature stringency and enables robust mutation enrichment.
BACKGROUND Low-level mutations in clinical tumor samples often reside below mutation detection limits, thus leading to false negatives that may impact clinical diagnosis and patient management. COLD-PCR (coamplification at lower denaturation temperature PCR) is a technology that magnifies unknown mutations during PCR, thus enabling downstream mutation detection. However, a practical difficulty ...
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BACKGROUND Multiplexed detection of low-level mutations presents a technical challenge for many technologies, including cancer gene panels used for targeted-resequencing. Analysis of mutations below approximately 2%-5% abundance in tumors with heterogeneity, samples with stromal contamination, or biofluids is problematic owing to increased noise from sequencing errors. Technologies that reduce ...
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Identifying low-abundance mutations within wild-type DNA is important in several fields of medicine, including cancer, prenatal diagnosis and infectious diseases. However, utilizing the clinical and diagnostic potential of rare mutations is limited by sensitivity of the molecular techniques employed, especially when the type and position of mutations are unknown. We have developed a novel platf...
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The successful isolation of Yersinia pseudotuberculosis from the stool of an asymptomatic family member of a patient with yersinia septicemia is presented. Cold enrichment permitted the isolation after 4 weeks of refrigerator incubatio,.
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2012
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2012.183095